subcellular localization prediction and analysis of pmc2h2 protein (CS Bio Inc)
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Subcellular Localization Prediction And Analysis Of Pmc2h2 Protein, supplied by CS Bio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Transcriptomic Identification of Potential C2H2 Zinc Finger Protein Transcription Factors in Pinus massoniana in Response to Biotic and Abiotic Stresses"
Article Title: Transcriptomic Identification of Potential C2H2 Zinc Finger Protein Transcription Factors in Pinus massoniana in Response to Biotic and Abiotic Stresses
Journal: International Journal of Molecular Sciences
doi: 10.3390/ijms25158361
Figure Legend Snippet: The characteristics of 57 C2H2 proteins identified and their subcellular localization prediction.
Techniques Used:
Figure Legend Snippet: Phylogenetic analysis and motif distribution of PmC2H2 ZFPs. Branches with different colors represented different subgroups. The subgroup was named from “I” to “V”.
Techniques Used:
Figure Legend Snippet: Sequences of the 10 motifs of PmC2H2 ZFPs.
Techniques Used:
Figure Legend Snippet: Subcellular localization analysis of PmC2H2-4 and PmC2H2-20 proteins in N. benthamiana leaves. The scale in the images is 20 μm. pCAMBIA-1302-mGFP5 was the control. DAPI—4′,6-diamidino-2-phenylindole, a blue fluorescent dye that shows DNA location. Chloroplast—chloroplast auto-fluorescence, displays the location of chloroplasts. GFP—green fluorescence protein, displays the location of the target protein. Bright—bright field. Merged—merged picture of four overlapped channels.
Techniques Used: Control, Fluorescence
Figure Legend Snippet: The figure above shows the expression levels of five PmC2H2 genes under different abiotic treatments, namely ( a ) ABA, ( b ) drought, ( c ) ETH, ( d ) H 2 O 2 , ( e ) mechanical damage, ( f ) MeJA, ( g ) NaCl, ( h ) PEG, and ( i ) SA. The absence of any significant difference is indicated by the presence of identical lowercase letters across different columns. Conversely, the presence of completely distinct lowercase letters between different columns signifies a statistically significant difference ( p < 0.05). In cases where multiple lowercase letters are present within the same column, it implies that there is no significant difference between that particular column and other columns containing any one of those lowercase letters. The relative expression at 0 h is normalized to “1”.
Techniques Used: Expressing
Figure Legend Snippet: Transcriptional activation assay of six PmC2H2 genes. Empty pGBKT7 vector was used as a negative control and pGBKT7- PmC3H20 was used as a positive control.
Techniques Used: Activation Assay, Plasmid Preparation, Negative Control, Positive Control